關於「NCBI Primer-BLAST」標籤，搜尋引擎有相關的訊息討論：

Primer designing tool - NCBI - NIH。

Finding primers specific to your PCR template (using Primer3 and BLAST). ... Use my own forward primer (5'->3' on plus strand).： 。

Publication - NCBI。

Primer-BLAST was developed at NCBI to help users make primers that are specific to intended PCR target. It uses Primer3 to design PCR primers and then uses ...： 。

Design PCR primers and check them for specificity - NCBI。

Go to the Primer BLAST submission form. Enter the target sequence in FASTA format or an accession number of an NCBI nucleotide sequence in the PCR Template ...： 。

Primer-Blast results - NCBI。

2 天前 · Making primers specific to your PCR template. more... Status, Failed Run again. Error, NCBI C++ Exception: Error: SERVICES(CNetScheduleException ...： 。

In silico assessment of primers for eDNA studies using PrimerTree ...。

2016年3月11日 · We amplified eDNA using 12 primer pairs targeting mammals, fish, ... Note that parallel queries to Primer-BLAST are queued by NCBI's servers ...。

A bioinformatics workflow for the evaluation of RT-qPCR primer ...。

1. A bioinformatics workflow using publicly available genomic resources and tools (NCBI Primer BLAST, in silico PCR in UCSC genome browser, and Ensembl DNA ...。

Pitfalls during in silico prediction of primer specificity for eDNA ...。

2020年7月16日 · Email; Facebook; Twitter; Linked In; Reddit; Wechat ... Primer pairs predicted by Primer-BLAST to be specific rarely showed such specificity ...。

MFEprimer: multiple factor evaluation of the specificity of PCR primers。

2008年11月27日 · Although a large number of PCR primer design programs have been ... 2007) only provide a link to the NCBI BLAST homepage for the user to ...。

CircPrimer: a software for annotating circRNAs and determining the ...。

2018年8月3日 · Furthermore, circPrimer help users to design primers for circRNAs and ... or the NCBI Primer Design tool to design primers for circRNA [28].。

Direct nucleic acid analysis of mosquitoes for high fidelity species ...。

2018年8月30日 · LAMP primer and OSD probe design. Wolbachia wAlbB and wPip strain wsp genes and Ae. aegypti coi gene sequences were obtained from NCBI GenBank.